RIBOZYME MEDIATED GENE THERAPY

Tissue-specific triple ribozyme vectors for prostate cancer gene therapy

Dale J. Voeks1, Gary A. Clawson2, and James S. Norris1 1Medical University of South Carolina, Charleston, SC; 2Penn State University, Hershey, PA __________________________________________________________________________________________________ Correspondence: Dr. James S. Norris, Department of Microbiology and Immunology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, Sou...

Vector - Mediated Cancer Gene Therapy

In recent years there has been a dramatic increase in developing gene therapy approaches for the treatment of cancer. The two events that have permitted the formulation of concept of cancer gene therapy are the new understanding of the molecular mechanisms underlying oncogenesis, and the development of the DNA-delivery vehicles or vectors. Many approaches to cancer gene therapy have been propos...

Ribozyme therapy: RNA enzymes to the rescue.

According to the central dogma of biology, ribonucleic acids (RNA)' were thought to play the role of messengers (mRNA) that are mere intermediates between DNA and proteins. Recent advances in understanding the capabilities of RNA have proven this wrong. The catalytic reaction required for making proteins is carried out by RNA [1]. RNA evolved in vitro can bind small molecules with high specific...

AAV Mediated β-Thalassemia Gene Therapy

Plasmid-mediated gene therapy for cardiovascular disease.

Gene transfer within the cardiovascular system was first demonstrated in 1989 yet, despite extensive basic-science and clinical research, unequivocal benefit in the clinical setting remains to be demonstrated. Potential reasons for this include the fact that recombinant viral vectors, used in the majority of clinical studies, have inherent problems with immunogenicity that are difficult to circ...